Invitrogen™ E. coli DNA Ligase

Invitrogen™ E. coli DNA Ligase

E. coli DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of β-NAD between double-stranded DNAs with 3´ hydroxyl and 5´ phosphate cohesive termini. Single-stranded nucleic acids are not substrates for this enzyme.

Contents

• E. coli DNA Ligase is supplied with a vial of 10X reaction buffer [188 mM Tris-HCl (pH 8.3)
• 906 mM KCl
• 46 mM MgCl2
• 37.5 mM DTT
• 1.5 mM λ-NAD
• 100 mM (NH4 )2 SO4 ]