Invitrogen™ Multi-Copy Pichia Expression Kit

Invitrogen™ Multi-Copy Pichia Expression Kit

The Multi-Copy Pichia Expression Kit contains the pPIC3.5K, pPIC9K, and pAO815 vectors for production and selection of Pichia strains that contain more than one copy of the gene of interest. In many cases, increased copy number has led to increased expression levels.

Contents

The Multi-Copy Pichia Expression Kit includes:

• pPIC3.5K, pPIC9K, and pAO815 vectors: 20 µg each 
• Pichia pastoris strains GS115 (his4), KM71 (arg4 his4 aox1::ARG4)
• Control strain GS115 Albumin
• Control strain GS115 β-gal
• Spheroplast Transformation Kit
• 5´ AOX1, 3´ AOX1: 2 µg each
• α-factor sequencing primers