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Invitrogen™ Image-iT™ Lipid Peroxidation Kit, for live cell analysis

On demand
Order number : C10445
(Ex-work price)
$ On demand

Invitrogen™ Image-iT™ Lipid Peroxidation Kit, for live cell analysis

 

The Image-iT™ Lipid Peroxidation Kit enables the detection of lipid peroxidation in live cells through oxidation of BODIPY™ 581/591 C11 reagent. This reagent localizes to membranes throughout live cells and upon oxidation by lipid hydroperoxides, displays a shift in peak fluorescence emission from ∼590 nm to ∼510 nm. Fluorescence from live cells shifts from red to green, providing a ratiometric indication of lipid peroxidation compatible with traditional and high content microscopy, as well as flow cytometry. This kit also includes cumene hydroperoxide as a positive control compound to induce lipid peroxidation in cells

 

Features

  • The reagent is provided as a stable, ready-to-use DMSO solution with a simple protocol compatible with standard workflows in fluorescence microscopy
  • The reagent is provided as five single-use vials containing sufficient total reagent for five 96-well plates or 100 coverslips

 

Contents

  • Image-iT® Lipid Peroxidation Sensor (Component A)
  • Cumene hydroperoxide (Component B)
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