Invitrogen™ Image-iT™ Lipid Peroxidation Kit, for live cell analysis
On demand
Order number :
C10445
Invitrogen™ Image-iT™ Lipid Peroxidation Kit, for live cell analysis
The Image-iT™ Lipid Peroxidation Kit enables the detection of lipid peroxidation in live cells through oxidation of BODIPY™ 581/591 C11 reagent. This reagent localizes to membranes throughout live cells and upon oxidation by lipid hydroperoxides, displays a shift in peak fluorescence emission from ∼590 nm to ∼510 nm. Fluorescence from live cells shifts from red to green, providing a ratiometric indication of lipid peroxidation compatible with traditional and high content microscopy, as well as flow cytometry. This kit also includes cumene hydroperoxide as a positive control compound to induce lipid peroxidation in cells
Features
- The reagent is provided as a stable, ready-to-use DMSO solution with a simple protocol compatible with standard workflows in fluorescence microscopy
- The reagent is provided as five single-use vials containing sufficient total reagent for five 96-well plates or 100 coverslips
Contents
- Image-iT® Lipid Peroxidation Sensor (Component A)
- Cumene hydroperoxide (Component B)
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