Invitrogen™ eBioscience™ BrdU Staining Buffer Set for Flow Cytometry

Invitrogen™ eBioscience™ BrdU Staining Buffer Set for Flow Cytometry

The BrdU Staining Buffer Set contains the optimized buffers needed for optimal BrdU staining with the BU20A monoclonal antibody for flow cytometric analysis. This set in combination with BrdU and DNase I steps allows for detection of BrdU pulse-labeled cells thereby enabling the examination of proliferating cells. Cycling cells are incubated with 5-bromo-2’-deoxyuridine (BrdU), a synthetic analog of thymidine which incorporates into newly synthesized genomic DNA during the S-phase of mitosis. Following DNA denaturation, the cells are stained for BrdU incorporation along with any other cell surface and/or intracellular targets of interest.

Contents

• BrdU Staining Buffer Concentrate (4X): 1 x 30 mL bottle; store at 2-8°C.
• Fixation/Permeabilization Diluent: 1 x 100 mL bottle; store at 2-8°C.