Invitrogen™ eBioscience™ BrdU Staining Kit for Flow Cytometry, FITC
On demandInvitrogen™ eBioscience™ BrdU Staining Kit for Flow Cytometry, FITC
The eBioscience BrdU Staining Kit for Flow Cytometry contains the
necessary reagents and buffers for identifying and examining
proliferating cells of mammalian species by flow cytometric analysis.
Cycling cells are incubated with 5-bromo-2’-deoxyuridine (BrdU), a
synthetic analog of thymidine which incorporates into newly synthesized
genomic DNA during the S-phase of mitosis. Following DNA denaturation,
the cells are stained for BrdU incorporation along with any other cell
surface and/or intracellular targets of interest.
This kit is optimized to achieve brighter staining compared to traditional protocols.
Reactivity/Species: Bovine, Canine, Cow, Human, Monkey, Mouse, Non-human primate, Rat, Rhesus.
Related Products
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- eBioscience™ Anti-Mouse CD3e Functional Grade Purified (145-2C11).
- eBioscience™ Anti-Mouse CD28 Functional Grade Purified (37.51).
- eBioscience™ Anti-Mouse CD4 APC (GK1.5).
- eBioscience™ Fixable Viability Dye eFluor™ 780.
Contents
- BrdU: 5 x 1 mL sterile vials; store at less than or equal to -70°C.
- DNase I: 10 x 0.3 mL vials; store at less than or equal to -70°C.
- Anti-BrdU FITC Antibody (clone BU20A): 1 x 100 test vial; store at 2-8°C.
- BrdU Staining Buffer Concentrate (4X): 1 x 30 mL bottle; store at 2-8°C.
- Fixation/Permeabilization Diluent: 1 x 100 mL bottle; store at 2-8°C.
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