Invitrogen™ LIVE/DEAD™ Cell Vitality Assay Kit, C12 Resazurin/SYTOX™ Green

Invitrogen™ LIVE/DEAD™ Cell Vitality Assay Kit, C12 Resazurin/SYTOX™ Green

The LIVE/DEAD Cell Vitality Assay Kit provides a simple, two-color fluorescence assay that distinguishes metabolically active cells from injured cells and dead cells. The assay is based on the reduction of C₁₂-resazurin to red-fluorescent C₁₂-resorufin in metabolically active cells and on the uptake of the cell-impermeant, green-fluorescent nucleic acid stain, SYTOX Green dye, in cells with compromised plasma membranes (usually late apoptotic and necrotic cells). In this assay, dead cells emit mostly green fluorescence and healthy, metabolically active cells emit mostly red fluorescence; injured cells exhibit reduced red and green fluorescence.

For Use With (Equipment): Fluorescence Microscope, Flow Cytometer, Microplate Reader

Contents

• 5 vials of C₁₂-resazurin (40 μg each vial)
• 1 vial of SYTOX™ green (100 μL, 10 μM solution in DMSO)
• 1 vial of DMSO (1.5 mL)
• 10X phosphate buffer (100 mL)