Invitrogen™ eBioscience™ BrdU Staining Kit for Flow Cytometry, PerCP-eFluor™ 710
On demandInvitrogen™ eBioscience™ BrdU Staining Kit for Flow Cytometry, PerCP-eFluor™ 710
The
eBioscience BrdU Staining Kit for Flow Cytometry contains the necessary
reagents and buffers for identifying and examining proliferating cells
of mammalian species by flow cytometric analysis. Cycling cells are
incubated with 5-bromo-2’-deoxyuridine (BrdU), a synthetic analog of
thymidine which incorporates into newly synthesized genomic DNA during
the S-phase of mitosis. Following DNA denaturation, the cells are
stained for BrdU incorporation along with any other cell surface and/or
intracellular targets of interest.
This kit is optimized to achieve brighter staining compared to traditional protocols.
Reactivity/Species: Bovine, Canine, Cow, Human, Monkey, Mouse, Non-human primate, Rat, Rhesus.
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Contents
- BrdU: 5 x 1 mL sterile vials; store at less than or equal to -70°C.
- DNase I: 10 x 0.3 mL vials; store at less than or equal to -70°C.
- Anti-BrdU PerCP-eFluor® 710 Antibody (clone BU20A): 1 x 100 test vial; store at 2-8°C.
- BrdU Staining Buffer Concentrate (4X): 1 x 30 mL bottle; store at 2-8°C.
- Fixation/Permeabilization Diluent: 1 x 100 mL bottle; store at 2-8°C.
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