Invitrogen™ eBioscience™ Human Intracellular (cytoplasmic/cytokine) Protein Flow Cytometry Workflow Kit

Invitrogen™ eBioscience™ Human Intracellular (cytoplasmic/cytokine) Protein Flow Cytometry Workflow Kit

The eBioscience Human Intracellular (cytoplasmic/cytokine) Protein Flow Cytometry Workflow Kit contains all the buffers, blockers, and beads to complete a standard flow cytometry experiment for staining intracellular cytoplasmic and cytokine targets in human cell suspensions.

Content

• UltraComp eBeads Plus Compensation Beads: 01-3333-41
• Fc Receptor Binding Inhibitor Polyclonal Antibody, eBioscience: 14-9161-71
• CellBlox Monocyte and Macrophage Blocking Buffer: B001T02F01
• eBioscience Intracellular Fixation & Permeabilization Buffer Set: 88-8824-00
• eBioscience Flow Cytometry Staining Buffer: 00-4222-57
• eBioscience Cell Stimulation Cocktail (plus protein transport inhibitors) (500X): 00-4975-93

When combined with the antibodies specific to your experiment, the reagents within this kit have been optimized to work together to detect cell surface targets by flow cytometry and broadly include:

• Effective compensation controls
• Fc receptor blocking
• Non-antibody, non-protein-based blocking solution for reducing background staining from fluorophores
• Antibody binding buffer that minimizes non-specific binding of antibodies
• Fixation buffer for storage of stained samples
• Activating cocktail to induce cytokine production