Invitrogen™ eBioscience™ Mouse Cytokine Positive Control Cells

Invitrogen™ eBioscience™ Mouse Cytokine Positive Control Cells

The Mouse Cytokine Positive Control Cells are recommended for use as positive control cells when establishing an intracellular cytokine staining protocol. Mouse splenocytes were stimulated with ConA (3 µg/mL) for two days, followed by recombinant IL-2 (20 ng/mL) and IL-4 (20 ng/mL) for three days. The cells were then restimulated with immobilized anti-mouse CD3 (10 µg/mL) and soluble anti-mouse CD28 (2 µg/mL) in the presence of Brefeldin A for 5 hours. These cells contain subpopulations that express IL-2, IL-3, IL-4, IL-10, GM-CSF, and IFNγ. The cells have been fixed with formaldehyde and are provided in a stabilizing buffer so that they may be stored at -80°C for up to six months or at 2-8°C for up to one month.