Invitrogen™ Gateway™ pET-DEST42 Vector

Invitrogen™ Gateway™ pET-DEST42 Vector

The Champion™ pET-DEST42 Gateway® destination vector is designed for rapid cloning with a Gateway® entry clone using lambda phage site-specific recombination and subsequent high-level prokaryotic expression controlled by the strong bacteriophage T7 promoter. Expression is induced by the production of T7 RNA polymerase in BL21(DE3) E. coli. 

Features

• Bacteriophage T7lac promoter for high-level expression
• O operator sequences for lac repressor binding to ensure tighter regulation of transcription
• pBR322 ori for minimal basal expression
• C-terminal V5 and 6xHis tags for efficient detection and purification
• R sites for efficient recombination with any attL-flanked Gateway® entry vector

Contents

• The Champion™ pET-DEST42 Gateway™ destination vector includes 6 µg of vector