Invitrogen™ Gateway™ pET-DEST42 Vector
On demand
Order number :
12276010
Invitrogen™ Gateway™ pET-DEST42 Vector
The Champion™ pET-DEST42 Gateway® destination vector is designed for rapid cloning with a Gateway® entry clone using lambda phage site-specific recombination and subsequent high-level prokaryotic expression controlled by the strong bacteriophage T7 promoter. Expression is induced by the production of T7 RNA polymerase in BL21(DE3) E. coli.
Features
- Bacteriophage T7lac promoter for high-level expression
- O operator sequences for lac repressor binding to ensure tighter regulation of transcription
- pBR322 ori for minimal basal expression
- C-terminal V5 and 6xHis tags for efficient detection and purification
- R sites for efficient recombination with any attL-flanked Gateway® entry vector
Contents
- The Champion™ pET-DEST42 Gateway™ destination vector includes 6 µg of vector
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