Invitrogen™ DES™-Inducible/Secreted Kit with pCoBlast

Invitrogen™ DES™-Inducible/Secreted Kit with pCoBlast

The DES®-Inducible/Secreted Kit provides the vector pMT/BiP/V5-His for inducible, secreted expression of recombinant proteins and a choice of Blasticidin (pCoBlast) or hygromycin (pCoHygro) selection. This vector offers the inducible metallothionein promoter that is induced upon addition of copper sulfate or cadmium chloride. The N-terminal signal sequence from the insect BiP gene is provided to direct the recombinant fusion protein through the secretory pathway of S2 cells into the culture medium. 

To facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the BiP signal sequence.

Features

• Small size (3.6 kb) to improve DNA yields and increase subcloning efficiency
• C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody
• C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin

Contents

• The DES™ Inducible/Secreted Kits contains 20 µg of each of a selection vector (pCoHygro or pCoBlast)
• pMT/BiP/V5-His A, B, C and pMT/BiP/V5-His/GFP
• Calcium Phosphate Transfection Kit
• forward and reverse primers (2 µg each)
• GIBCO™ Schneider's Drosophila Medium
• frozen S2 cells
• the appropriate selection agent.