Invitrogen™ GeneArt™ CRISPR Nuclease mRNA

Invitrogen™ GeneArt™ CRISPR Nuclease mRNA

GeneArt™ CRISPR Nuclease mRNA is formulated using a new manufacturing process to achieve superior quality and improved product performance. This wild-type Cas9 mRNA has two nuclear localization signals (NLSs). Cas9 constructs with two NLSs have been shown to be more efficient at targeting the Cas9 protein to the nucleus (1). The mRNA format is ready to transfect and circumvents the need for time-consuming cloning steps required when using CRISPR vector systems. Cas9 mRNA is co-transfected with a target-specific guide RNA (gRNA) to direct the Cas9 protein to the intended genome locus to create a double-stranded break. The complete RNA format is a smaller payload than plasmid-based Cas9 systems for improved delivery into the cell and better genome editing efficiency. Additionally, the Cas9 mRNA can be used in multiplex approaches with more than one gRNA. Use this approach to determine which gRNA sequence works best for a particular target or to edit multiple genomic loci with one transfection.

Contents

• 1 vial CRISPR Nuclease mRNA