Invitrogen™ GeneArt™ Seamless Cloning and Assembly Enzyme Mix

Invitrogen™ GeneArt™ Seamless Cloning and Assembly Enzyme Mix

GeneArt® Seamless Cloning and Assembly Enzyme Mix enables the simultaneous and directional cloning of 1 to 4 PCR fragments, consisting of any sequence, into any linearized vector, in a single 30-minute room temperature reaction. GeneArt® Seamless enzyme mix is the economical choice for creating constructs up to 13 kb with the option for high-throughput assembly.

Features

• Efficiency: pre-cloning option for large fragments for increased cloning efficiency
• Speed and Ease: clone DNA fragments, with sequence of your choice, in a single vector (up to 13 kb); no restriction digestion, ligation, or recombination sites required
• Free Tools: design of your final construct and DNA oligos in silico using our free web-based tool that takes you step-by-step through your project
• Vector Flexibility: use our linear vector or a vector of your choice

Contents

• 1 vial GeneArt Enzyme Mix (2X)
• pUC19L Linearized Vector
• Control Insert