Invitrogen Granzyme B Monoclonal Antibody (NGZB), PE-Cyanine5.5, 100 µg, eBioscience™
On demandInvitrogen Granzyme B Monoclonal Antibody (NGZB), PE-Cyanine5.5, 100 µg, eBioscience™
This NGZB monoclonal antibody reacts with mouse Granzyme B, which is a member of the granzyme serine protease family. Granzyme B is found in the granules of cytotoxic T cells and NK cells. Granzyme B has also been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved has been identified as mannose 6-phosphate receptor. This receptor functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis. This NGZB monoclonal antibody does not crossreact to human Granzyme B nor is staining blocked with GB11, suggesting it recognizes a different epitope.
Features
- Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
- Fixation: Samples can be stored in IC Fixation Buffer (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
- Excitation: 488-561 nm; Emission: 695 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
- Recommended Isotype Control: Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine5.5, eBioscience™.
There are no specifications
There are no report
You May Also Like
Invitrogen IL-1 beta (Pro-form) Monoclonal Antibody (NJTEN3), PerCP-eFluor 710, 100 µg, eBioscience™
$ On demand
On demand
Invitrogen 25 µg Ki-67 Monoclonal Antibody (SolA15), PE, eBioscience™
$ On demand
On demand
Invitrogen CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), Unconjugated, 500 µg, eBioscience™
$ 222
On demand
Invitrogen CD45 Monoclonal Antibody (30-F11), APC-eFluor 780, 25 µg, eBioscience™
$ On demand
On demand
Invitrogen 500 µg Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™
$ On demand
On demand
Invitrogen CD40 Monoclonal Antibody (1C10), Biotin, 500 µg, eBioscience™
$ On demand
On demand
Invitrogen Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 555
$ On demand