Invitrogen HGF-Cell Lysates Mouse ELISA Kit, 10 x 96 Tests

Invitrogen HGF-Cell Lysates Mouse ELISA Kit, 10 x 96 Tests

The Mouse Hepatocyte Growth Factor(Ms HGF) Cell Lysates ELISA quantitates Ms HGF in mouse cell or tissue lysates. The assay will exclusively recognize both natural and recombinant Ms HGF.
Principle of the method
The Mouse HGF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features  

• Homogenous (no wash): No    
• Interassay CV: <12%
• Intraassay CV: <10%
• Shipping condition: Wet or Dry Ice
• Storage: 2-8°C

Contents    

• Mouse HGF Antibody Coated wells, 96-well plate, 10 plate
• Mouse HGF Biotin Conjugate, 20 vials
• Mouse HGF Standard, recombinant mouse HGF, 20 vials
• Wash Buffer Concentrate (20X), 12 x 25 mL
• Sample Diluent Buffer (5X), 10 x 10 mL
• Assay Diluent (5X), 10 x 15 mL
• Cell lysate buffer (2X), 10 x 5 mL
• Streptavidin-HRP (500X), 10 x 0.2 mL
• TMB Substrate, 10 x 12 mL
• Stop Solution, 10 x 8 mL
• Adhesive Plate Covers, 20