Invitrogen™ Histone Demethylase Fluorescent Activity Kit

Invitrogen™ Histone Demethylase Fluorescent Activity Kit

The Histone Demethylase Activity research-use-only kit is a fluorescent activity assay designed for the quantification and detection of histone demethylase activity of lysine-specific histone demethylase 1 (LSD1)-and Jumonji-type demethylases.

This kit includes black 96-well plate(s), LSD1/JMJD2A assay buffers, formaldehyde standard, and other components to perform the assay. This kit does not contain demethylase enzyme samples. A source of LSD1-type or Jumonji-type demethylase, along with any cofactors, enzyme substrates, inhibitors, and/or activators need to be supplied by the user. A fluorescence 96-well microplate reader capable of reading fluorescent emission at 510 nm, with excitation at 450 nm, is required for use of this kit.

Features

• Storage: Store at –20°C.
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Target Kit Named: Histone Demethylase
• Sample types: lysine-specific histone demethylase 1 (LSD1) and Jumonji-type demethylases
• Reactivity: human
• Standard curve range: 0.128 uM–0.64 uM for LSD1, 2.5 uM-10uM for JMJD2A
• For Use With (Equipment): Microplate Reader

Content

• Formaldehyde Standard; 2,000 μM formaldehyde solution in a special stabilizing solution, keep tightly sealed, 500 μL
• LSD1-type Assay Buffer; phosphate buffer containing detergents and stabilizers, 60 mL
• JMJD2A-type Assay Buffer; HEPES buffer containing stabilizers, 60 mL
• Black 96-well Half Area Plate 2 plates
• Formaldehyde Reagent; contains 0.09% sodium azide as a preservative 5 mL
• Demethylase Cell Lysis Buffer; Tris based buffer containing detergents, store frozen 100 mL
• Plate sealer 2