Invitrogen™ P450 Demethylation Fluorescent Activity Kit

Invitrogen™ P450 Demethylation Fluorescent Activity Kit

The P450 Demethylase Activity research-use-only kit is a fluorescent activity assay designed for the quantification and detection of P450 demethylating actrivity in liver microsomes or cerosomes such as Cyp P450 3A4, 2B4 and 2D6.

This kit includes black 96-well plate(s), assay buffer, formaldehyde standard, and other components to perform the assay. This kit does not contain P450 systems. Microsome, cerosome, baculosome, or supersome P450 systems, or recombinant P450, need to be supplied by the user. A fluorescence 96-well microplate reader capable of reading fluorescent emission at 510 nm, with excitation at 450 nm, is required for use of this kit.

Features

• Sample types: demethylating P450 systems like liver microsomes or cerosomes such as Cyp P450 3A4, 2B4, and 2D6
• Standard curve range: 1.6 pmoles/100uL–20 pmoles/100uL
• Reactivity: human, rat
• Storage: Store at –20°C.
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Target Kit Named: P450 Demethylation
• For Use With (Equipment): Microplate Reader

Content

• Formaldehyde Standard; 2,000 μM formaldehyde solution in deionized water, keep tightly sealed 500 μL
• Assay Buffer; 100 mM potassium phosphate buffer at pH 7.4 containing 0.005% gentamicin 60 mL
• Black 96-well Half Area Plate 2 plates
• NADPH lyophilized; reduced ß-nicotinamide adenine dinucleotide 2’-phosphate (NADPH) with stabilizers 2 vials
• Formaldehyde Reagent; contains 0.09% sodium azide as a preservative 5 mL
• Stop Solution; 1M solution of acetic acid in water, CAUTION 1 mL
• Plate Sealer 2