Invitrogen MCP-5 (CCL12) Mouse ELISA Kit, 96 Tests
On demandInvitrogen MCP-5 (CCL12) Mouse ELISA Kit, 96 Tests
The Mouse MCP-5 (CCL12) ELISA quantitates Ms MCP-5 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms MCP-5.
Principle of the method
The Mouse MCP-5 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Features
- Homogenous (no wash): No
- Interassay CV: <12%
- Intraassay CV: <10%
- Shipping condition: Wet or Dry Ice
- Storage: 2-8°C
Contents
- Mouse MCP-5 Antibody Coated wells, 96-well plate, 1 plate
- Mouse MCP-5 Biotin Conjugate, 2 vials
- Mouse MCP-5 Standard, recombinant mouse MCP-5, 2 vials
- Wash Buffer Concentrate (20X), 25 mL
- Assay Diluent A (contains 0.09% sodium azide), 30 mL
- Assay Diluent B (5X), 15 mL
- Streptavidin-HRP (160X), 0.2 mL
- TMB Substrate, 12 mL
- Stop Solution, 8 mL
- Adhesive Plate Covers, 2