Invitrogen IL-20 Human ELISA Kit, 10 x 96 Tests

Invitrogen IL-20 Human ELISA Kit, 10 x 96 Tests

The Human Interleukin 20 (Hu IL-20) ELISA quantitates Hu IL-20 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-20.
Principle of the method
The Human IL-20 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

• Homogenous (no wash): No    
• Interassay CV: <12%
• Intraassay CV: <10%

Contents    

• Human IL-20 Antibody Coated wells, 96-well plate, 10 plate
• Human IL-20 Biotin Conjugate, 20 vials
• Human IL-20 Standard, recombinant human IL-20, 20 vials
• Wash Buffer Concentrate (20X), 12 x 25 mL
• Assay Diluent C, 10 x 30 mL
• Assay Diluent B (5X), 10 x 15 mL
• Streptavidin-HRP (500X), 10 x 0.2 mL
• TMB Substrate, 10 x 12 mL
• Stop Solution, 10 x 8 mL
• Adhesive Plate Covers, 20