Invitrogen™ LIVE/DEAD™ Cell-Mediated Cytotoxicity Kit, for animal cells
On demand
Order number :
L7010
Invitrogen™ LIVE/DEAD™ Cell-Mediated Cytotoxicity Kit, for animal cells
The LIVE/DEAD™ Cell-Mediated Cytotoxicity Kit
measures natural killer (NK) cell-mediated, lymphokine-activated killer
(LAK) cell-mediated and T cell mediated cytotoxicity. Target cells are
preincubated with the green-fluorescent membrane stain DiOC18
and then mixed with effector cells in the presence of the
red-fluorescent, membrane-impermeant dye, propidium iodide. Live and
dead target cells retain the green-fluorescent membrane stain; target
and effector cells with compromised membranes exhibit red-fluorescent
nucleic acid staining; live effector cells are nonfluorescent.
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