Invitrogen™ SYTOX™ Orange Dead Cell Stain, for flow cytometry

Invitrogen™ SYTOX™ Orange Dead Cell Stain, for flow cytometry

SYTOX™ Orange Dead Cell Stain is a bright, easy-to-use nucleic acid stain for distinguishing dead from live cells in flow cytometry assays. It is excitable at 532 nm with an emission profile similar to R-PE, and has been tested in an assortment of cell types.

Features

• A bright stain in an easy-to-use, no-wash assay, SYTOX™ Orange Dead Cell Stain is a cell-impermeant nucleic acid stain. By flow cytometry, dead cells can be easily distinguished from live cells by the increased fluorescent signal due to dsDNA binding of SYTOX™ Orange. Because it is non-fluorescent in aqueous media, SYTOX™ Orange Dead Cell Stain can be applied to cells and visualized with minimal background staining and without an additional wash step.
• Validated in flow cytometry, SYTOX™ Orange Dead Cell Stain is designed for flow cytometry using the 488 nm blue laser or 532 nm green laser with emission in the 575/26 BP filter (similar to PE). The dye concentration has been optimized for samples with concentrations ranging from 1x10⁴to 1x10⁶ cells per ml.
• Validation, in Jurkat, ReH, CHO, Kg1a, 3T3, HeLa, HEK (primary cells), and lysed whole blood.
• Tested in several applications including, Apoptosis, Cell cycle, Cell proliferation, Immunophenotyping assays.

Contents

• 1 vial of SYTOX™ orange dead cell stain (250 μM solution in DMSO).