Invitrogen™ LIVE/DEAD™ Viability/Cytotoxicity Assay Kit (Green/Deep Red)

Invitrogen™ LIVE/DEAD™ Viability/Cytotoxicity Assay Kit (Green/Deep Red)

The LIVE/DEAD Viability/Cytotoxicity Assay Kit (Green/Deep Red) is a two-color fluorescent cell viability assay that simultaneously identifies live and dead cells. Live cells are labeled with calcein dye and emit a green fluorescence, while dead cells are labeled with SYTOX Deep Red dye and emit a red fluorescence. These dyes can be multiplexed with other orange/red fluorophores with compatible filter sets

Features

• Determines cellular viability with two-color fluorescence
• Detect live cells with GFP/FITC filter set and dead cells with Deep Red/CY5 filter set
• Multiplexable with orange/red fluorophores such as RFP, mCherry, Mitotracker Red/Orange, Lysotracker Red, TMRM, and other
• For Use With (Equipment): Fluorescence Microscopes or High Content Analysis (HCA) Multi-Well Plate Fluorescence Scanners or Other Fluorescence Detection Systems

Contents

• 1 vial of Calcein AM (live cell indicator), 40 µL, provided as a 2000X (4 mM) solution in DMSO
• 1 vial of SYTOX Deep Red Nucleic Acid Stain (dead cell indicator), 40 µL, provided as a 2000X (500 µM) solution in DMSO