Invitrogen™ MultiShot™ FlexPlate DH5α T1R Competent Cells

Invitrogen™ MultiShot™ FlexPlate DH5α T1R Competent Cells

MultiShot FlexPlate DH5α T1R chemically competent E. coli cells are cloning competent cells that are pre-aliquoted in a 96-well PCR plate to increase transformation productivity. The FlexPlate format can be divided down to any combination of 8-well segments for flexibility in transformation throughput.

MultiShot FlexPlate DH5α T1R competent E. coli cells are derived from the popular DH5α strain, one of the most frequently used laboratory cloning strains. All of our T1R strains have the tonA (fhuA) deletion in their genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyse E. coli hosts, which are commonly used for cloning and library construction. A resistant genotype offers added security to protect valuable clones and libraries. This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic.

Features

• hsdR for efficient transformation of unmethylated DNA from PCR amplifications
• lacZΔM15 for blue/white color screening of recombinant clones
• endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by endonuclease I
• recA1 for reduced occurrence of non-specific recombination in cloned DNA
• tonA (fhuA) for T1 and T5 phage resistance

Contents

• MultiShot FlexPlate DH5α T1R Competent Cells: One 96-well plate, 20 µL per well
• pUC19 DNA (10 pg/µL): 1 vial, 50 µL
• S.O.C. medium: 2 bottles, 10 mL each