Invitrogen™ One Shot™ INV110 Chemically Competent E. coli

Invitrogen™ One Shot™ INV110 Chemically Competent E. coli

INV110 E. coli are derived from JM110 E. coli and are specifically designed for the growth and purification of plasmid DNA that will be digested with dam or dcm methylation-sensitive restriction enzymes. INV110 is a significant improvement over the JM110 strain. INV110 has been engineered to carry an endA1 mutation, which eliminates the non-specific endonuclease Endonuclease I, for improved plasmid DNA preparation.

Features

• Blue/white screening of recombinant clones (lacZΔM15)
• Reduced cleavage of foreign methylated DNA for improved transformation of genomic DNA [Δ(mcrC-mrr)]
• F´ episome for phage infection and rescue of single-strand DNA

Contents

• One Shot™ INV110 Chemically Competent E. coli contains 10 transformations of chemically competent cells in One Shot™ format