Invitrogen™ One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E. coli
On demand
Order number :
C862003
Invitrogen™ One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E. coli
One Shot® Mach1™ T1 Phage-Resistant Chemically Competent E. coli are the fastest growing chemically competent cells currently available (see figure). Doubling time is approximately 50 minutes, compared to >74 minutes for other cloning strains. Mach1™ colonies are clearly visible within eight hours of plating the transformation mix, enabling you to plate and pick colonies the same day. Perform mini-preps after only 4 hours of growth from an overnight colony—saving an entire day in your experiments
Features
- Minimizes nonspecific recombination in cloned DNA (recA)
- Offers resistance to T1 and T5 phage (tonA)
- Yields cleaner preparations of DNA and better results in downstream applications due to the elimination of nonspecific digestion by Endonuclease I (endA1)
- Provides efficient transformation of unmethylated DNA from PCR amplifications (hsdR)
- Allows blue/white screening of recombinant clones (lacZΔM15)
Contents
- One Shot™ Mach1™-T1R Chemically Competent E. coli: 21 vials, 50 µl each
- pUC19 DNA (10 pg/µl): 1 vial, 50 µl
- S.O.C. medium: 1 bottle, 6 ml
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