Invitrogen™ MultiShot™ FlexPlate OmniMAX™ 2 T1R Competent Cells

Invitrogen™ MultiShot™ FlexPlate OmniMAX™ 2 T1R Competent Cells

MultiShot FlexPlate OmniMAX 2 T1R chemically competent E. coli cells are a superior strain of cloning competent cells that are pre-aliquoted in a 96-well PCR plate to increase transformation productivity. The FlexPlate format can be divided down to any combination of 8-well segments for flexibility in transformation throughput.

All of our T1R strains have the tonA (fhuA) deletion in their genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyse E. coli hosts, which are commonly used for cloning and library construction. A resistant genotype offers added security to protect valuable clones and libraries. This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic.

Features

• Δ(ccdAB) for sensitivity to the toxic effects of the ccdB gene product, allowing negative selection of vectors containing the ccdB gene—ideal for transformation of Gateway and TOPO reactions
• mcrA, mcrBC, mrr, and hsdRMS elimination of restriction systems to allow construction of more representative genomic constructs
• tonA (fhuA) genotype to confer resistance to T1 and T5 phage
• Highest transformation efficiency in FlexWell format (>5x108 cfu/µg control DNA)
• endA1 for increased plasmid yield and quantity
• recA1 for reduced occurrence of non-specific recombination in cloned DNA
• lacZΔM15 for blue/white color screening of recombinant clones

Contents

• MultiShot FlexPlate OmniMAX 2 T1R Competent Cells: One 96-well plate, 20 µL per well
• pUC19 DNA (10 pg/µL): 1 vial, 50 µL
• S.O.C. medium: 2 bottles, 10 mL each