Invitrogen™ MultiShot™ StripWell DH5α T1R Competent Cells

Invitrogen™ MultiShot™ StripWell DH5α T1R Competent Cells

MultiShot StripWell DH5α T1R chemically competent E. coli cells are cloning competent cells that are packaged in a rack containing 12 strips of 8 wells to increase productivity for medium-throughput bacterial transformations. MultiShot StripWell DH5α T1R competent E. coli cells are derived from the popular DH5α strain, one of the most frequently used laboratory cloning strains. All of our T1R strains have the tonA (fhuA) deletion in their genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyse E. coli hosts, which are commonly used for cloning and library construction. A resistant genotype offers added security to protect valuable clones and libraries. This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic.

Features

• hsdR for efficient transformation of unmethylated DNA from PCR amplifications
• lacZΔM15 for blue/white color screening of recombinant clones
• endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by endonuclease I
• recA1 for reduced occurrence of non-specific recombination in cloned DNA
• tonA (fhuA) for T1 and T5 phage resistance

Contents

• MultiShot StripWell DH5α T1R Competent Cells: One 96-stripwell rack, 50 µL per well
• pUC19 DNA (10 pg/µL): 1 vial, 50 µL
• S.O.C. medium: 2 bottles, 15 mL each