Invitrogen™ MultiShot™ StripWell BL21 Star™ (DE3) Competent Cells

Invitrogen™ MultiShot™ StripWell BL21 Star™ (DE3) Competent Cells

MultiShot StripWell BL21 Star (DE3) chemically competent E. coli cells are cloning competent cells that are packaged in a rack containing 12 strips of 8 wells to increase productivity for medium-throughput bacterial transformations. MultiShot StripWell BL21 Star (DE3) competent E. coli cells are designed for applications that require high-level expression of non-toxic recombinant proteins from low copy number, T7 promoter-based expression systems (e.g., Champion pET vectors). MultiShot StripWell BL21 Star (DE3) Competent Cells are provided at a transformation efficiency of 1 x 107 cfu/µg plasmid DNA.

Features

• rne131 for promotion of high mRNA stability and protein yield
• OmpT and lack of lon for minimal degradation of heterologous proteins by proteases
• Optimized for use with low copy number, T7 promoter-based plasmids

Contents

• MultiShot StripWell BL21 Star (DE3) Competent Cells: One 96-stripwell rack, 50 µL per well
• pUC19 DNA (10 pg/µL): 1 vial, 50 µL
• S.O.C. medium: 2 bottles, 15 mL each