Invitrogen™ One Shot™ MAX Efficiency™ DH5α-T1R Competent Cells

Invitrogen™ One Shot™ MAX Efficiency™ DH5α-T1R Competent Cells

One Shot MAX Efficiency DH5α-T1R Competent Cells are derived from the popular DH5α strain. DH5α-T1R carry the tonA genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyse E. coli hosts, which are commonly used for cloning and library construction. They offer added security to protect valuable clones and libraries. This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic.

Features

• Cleaner preparations of DNA and better results in downstream applications due to the elimination of nonspecific digestion by Endonuclease I (endA1)
• Reduced occurrence of unwanted recombination in cloned DNA (recA1)
• Efficient transformation of unmethylated DNA from PCR applications (hsdR)
• Blue/white color screening of recombinant clones carrying the alpha fragment of β-galactosidase (lacZΔM15)

Contents

• One Shot MAX Efficiency DH5α-T1R Competent Cells: 20 vials, 50 µl each ( total of 1 ml)
• pUC19 DNA (10 pg/ul): 1 vial, 50 µl
• S.O.C. medium: 1 bottle, 6 ml