Invitrogen™ MultiShot™ StripWell TOP10 Chemically Competent E. coli, 1 Plate

Invitrogen™ MultiShot™ StripWell TOP10 Chemically Competent E. coli, 1 Plate

MultiShot StripWell TOP10 chemically competent E. coli cells are cloning competent cells that packaged in a rack containing 12 strips of 8 tubes to increase productivity for medium-throughput bacterial transformations. TOP10 competent E. coli cells are ideal for high-efficiency cloning and plasmid propagation. They allow stable replication of high-copy number plasmids and are the same competent cells that come with many of our cloning kits.

Features

• hsdR for efficient transformation of unmethylated DNA from PCR amplifications
• mcrA for efficient transformation of methylated DNA from genomic preparations
• lacZΔM15 for blue/white color screening of recombinant clones
• endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by endonuclease I
• recA1 for reduced occurrence of non-specific recombination in cloned DNA

Contents

• MultiShot™ StripWell TOP10 Chemically Competent E. coli: One 96-stripwell plate, 50 µl per well
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• S.O.C. medium: 2 bottles, 15 ml each