Invitrogen™ MultiShot™ StripWell Stbl3™ Competent Cells

Invitrogen™ MultiShot™ StripWell Stbl3™ Competent Cells

MultiShot StripWell Stbl3 chemically competent E. coli cells are cloning competent cells that are pre-aliquoted in a rack containing 12 strips of 8 wells to increase productivity for medium-throughput bacterial transformations. MultiShot StripWell Stbl3 competent E. coli cells are designed especially for cloning direct repeats found in lentiviral expression vectors. Unlike TOP10 E. coli, these cells reduce the frequency of homologous recombination of long terminal repeats (LTRs) found in ViraPower Lentiviral Expression vectors and other retroviral vectors. The DNA yield from Stbl3 cells is often >10-fold higher than alternative strains such as TOP10.

Features

• Optimal performance for the propagation of lentiviral vectors
• mcrB, mrr for enhanced genomic cloning capacity
• recA13 for reduced occurrence of non-specific recombination in cloned DNA
• High DNA yields

Contents

• MultiShot StripWell Stbl3 Competent Cells: One 96-stripwell rack, 50 µL per well
• pUC19 DNA (10 pg/µL): 1 vial, 50 µL
• S.O.C. medium: 2 bottles, 15 mL each