Invitrogen™ One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli

Invitrogen™ One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli

The One Shot® OmniMAX™ 2 T1R E. coli strain is an improved chemically competent cell line, perfect for use in all cloning applications, including the Gateway® technology. OmniMAX™ 2 T1R cells offer the highest transformation efficiency of any chemically competent cells in a One Shot® format, with >5 x 10⁹ transformants/µg pUC19. They also provide efficient transformation of highly methylated DNA, since OmniMAX™ 2 T1R cells lack the E. coli K12 restriction systems (mcrA Δ(mrr hsdRMS-mcrBC)). In addition, the strain carries the tonA genotype, which confers resistance to T1 and T5 phage infection. This protects your samples and minimizes the possibility of downtime in your lab due to phage contamination.

Features

• Ideal for transformation of Gateway® and TOPO® reactions
• Resistance to T1 and T5 phage (tonA)
• Construction of more representative genomic libraries due to the elimination of mcrA, mcrBC, mrr, and hsdRMS
• Blue/white screening of recombinant clones (lacZΔM15)

Contents

• One Shot™ OmniMAX™ 2 T1R E. coli: 21 vials, 50 µl each
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• S.O.C. medium: 1 bottle, 6 ml