Invitrogen™ MaV203 Competent Yeast Cells, Subcloning Scale

Invitrogen™ MaV203 Competent Yeast Cells, Subcloning Scale

MaV203 Competent Yeast Cells are designed for use with the ProQuest™ Two-Hybrid System (Figure 1). S. cerevisiae strain MaV203 contains deletions in the endogenous GAL4 and GAL80 genes for use with GAL4-based two-hybrid systems. The strain also has leu2 and trp1 mutations for selection of ProQuest™ bait and prey vectors. MaV203 contains three GAL4 inducible reporter genes, providing four phenotypes, for easier identification of true interactors. 

Feature

• Transformation (250 µl of cells) efficiency yields >1 x 106 colonies
• Control DNA pMAB12 to confirm transformation efficiency and the preparation of selection media
• Pre-qualified PEG/Lithium Acetate to maximize number of trans-formants
• Competent cells formatted with carrier DNA already included

Contents

• MaV203 Competent Yeast Cells: 4 x 100 μL 
• PEG/Lithium Acetate (LiAc) Solution: 2 x 1.5 mL 
• pMAB12 DNA, 20 ng/μL: 10 μL