Invitrogen™ MaV203 Competent Yeast Cells, Subcloning Scale
On demand
Order number :
11445012
Invitrogen™ MaV203 Competent Yeast Cells, Subcloning Scale
MaV203 Competent Yeast Cells are designed for use with the ProQuest™ Two-Hybrid System (Figure 1). S. cerevisiae strain MaV203 contains deletions in the endogenous GAL4 and GAL80 genes for use with GAL4-based two-hybrid systems. The strain also has leu2 and trp1 mutations for selection of ProQuest™ bait and prey vectors. MaV203 contains three GAL4 inducible reporter genes, providing four phenotypes, for easier identification of true interactors.
Feature
- Transformation (250 µl of cells) efficiency yields >1 x 106 colonies
- Control DNA pMAB12 to confirm transformation efficiency and the preparation of selection media
- Pre-qualified PEG/Lithium Acetate to maximize number of trans-formants
- Competent cells formatted with carrier DNA already included
Contents
- MaV203 Competent Yeast Cells: 4 x 100 μL
- PEG/Lithium Acetate (LiAc) Solution: 2 x 1.5 mL
- pMAB12 DNA, 20 ng/μL: 10 μL
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