Invitrogen™ One Shot™ TOP10 Electrocomp™ E. coli, 11 Tubes

Invitrogen™ One Shot™ TOP10 Electrocomp™ E. coli, 11 Tubes

One Shot® TOP10 Electrocomp™ E. coli are provided at a transformation efficiency of 1 x 1010 cfu/ µg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation. They allow stable replication of high copy number plasmids

Features

• hsdR for efficient transformation of unmethylated DNA from PCR amplifications
• mcrA for efficient transformation of methylated DNA from genomic preparations
• lacZΔM15 for blue/white color screening of recombinant clones
• endA1 for cleaner preparations of DNA—get better results in downstream applications due to the elimination of nonspecific digestion by Endonuclease I
• recA1 for reduced occurrence of nonspecific recombination in cloned DNA

Contents

• One Shot™ TOP10 Electrocomp™ E. coli: 21 vials, 50 µl each
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• S.O.C. medium: 1 bottle, 6 ml