Invitrogen™ Pacific Blue™ Antibody Labeling Kit

Invitrogen™ Pacific Blue™ Antibody Labeling Kit

Molecular Probes™ Antibody Labeling Kits provide a convenient means to label small amounts of antibodies with the violet light-excitable Pacific Orange™ or Pacific Blue™ dyes. This kit is optimized for labeling 100 µg of antibody per reaction. Comparably small amounts of other proteins (>40 kDa) can also be labeled.

Features

• Pacific Blue™ has an excitation and emission maximum of 410/455 nm
• Labeled proteins typically ready to use in 90 min (∼15 min hands-on time)
• Useful for labeling 100 µg of protein
• Optimized for small-scale labeling of any protein >40 kDa
• Purified using convenient spin filters
• Stabilizing proteins must be removed from the sample before labeling
• Includes detailed instructions for determining degree of labeling (DOL) 

Contents

• Contains 5 vials of Pacific Blue™ reactive dye, 1 vial sodium bicarbonate (84 mg), purification resin (10 mL), 5 spin columns, and and 5 collection tubes.