Thermo Scientific™ Pierce™ Biotin Quantitation Kit

Thermo Scientific™ Pierce™ Biotin Quantitation Kit

Thermo Scientific Pierce Fluorescence Biotin Quantitation Kit requires only ten microliters of sample to accurately measure the biotinylation level of labeled antibodies and other biomolecules.

HABA dye (4'-hydroxyazobenzene-2-carboxylic acid) is a reagent that enables quick estimation of the mole-to-mole ratio of biotin to protein in a solution. The Pierce Biotin Quantitation Kit contains a premix of HABA and avidin and a biotinylated horseradish peroxidase (HRP) positive control. The HABA-Avidin Premix is supplied in convenient Thermo Scientific No-Weigh Microtube packaging, which eliminates the difficulties associated with weighing small quantities of reagent.

Features

• Colorimetric – requires a plate reader or spectrophotometer set to measure at 500nm
• Reliable – proven, well-characterized method for determination of biotinylation levels
• Convenient – kit provides the essential reagents and standards to perform the assay with ease
• Flexible – adaptable to spectrophotometer cuvettes or standard plate readers with 96-well microplates
• Options – calculate results directly from absorbance values based on extinction coefficients using the procedure outlined in the instructions
• Robust – supplied HABA-avidin complex can be used over a wide range of pH and salt concentrations

Contents

Sufficient For: Testing biotinylation of 12 protein samples

• HABA-Avidin Premix: 24 tubes
• Biotinylated Horseradish Peroxidase: 5 mg