Invitrogen™ pBAD/TOPO™ ThioFusion™ Expression Kit

Invitrogen™ pBAD/TOPO™ ThioFusion™ Expression Kit

The pBAD/TOPO® ThioFusion™ Expression Kit is designed for one-step cloning and regulated expression of thioredoxin fusion proteins in E. coli(Figure 1). The pBAD/Thio-TOPO® vector encodes His-Patch thioredoxin as an N-terminal fusion partner. The thioredoxin fusion can significantly increase the solubility of many difficult-to-express proteins and improve the yield of protein production.

Features:

• The araBAD promoter for tightly regulated expression in E. coli I-activated vector for 5-minute TOPO® Cloning of Taq-amplified PCR products
• C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin and detection with an Anti-His(C-term) Antibody
• C-terminal V5 epitope for detection with an Anti-V5 Antibody
• Enterokinase cleavage site for efficient cleavage of N-terminal fusion tag with EKMax™ Enterokinase

Contents

• pBAD/Thio-TOPO® vector, linearized: 20 reactions 
• 10X PCR Buffer: 100 μL
• dNTP Mix: 10 μL
• Salt Solution: 50 μL
• 20% L-Arabinose: 1 mL
• Trx Forward Sequencing Primer: 20 μL
• pBAD Reverse Sequencing Primer: 20 μL
• Control PCR Primers: 10 μL
• Control PCR Template: 10 μL