Invitrogen™ GeneArt™ Gibson Assembly EX Master Mix, 50 Reactions

Invitrogen™ GeneArt™ Gibson Assembly EX Master Mix, 50 Reactions

GeneArt Gibson Assembly EX Master Mix enables DNA assembly via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction. DNA fragments of different lengths are uniformly assembled using complementary overlaps between fragments. The inherent flexibility and success of this approach is suitable for small and large DNA constructs, includes both single and multiple inserts, and has been used to build entire genomes. The resulting products can be used for a variety of downstream applications, including transformation, PCR, and rolling circle amplification (RCA). The GeneArt Gibson Assembly EX Master Mix kit includes master mix, positive control, and water, and accommodates the use of your own competent cells.

Features

Simple—seamlessly assemble and clone up to 15 DNA fragments in a single reaction

Efficient—robust efficiency provides successful clones for both simple and challenging constructs

Flexible—design guidelines allow assembly into any vector of your choice

Convenient—available as master mix kits and cloning kits complete with chemical or electrocompetent cells

Trusted—over 4000 citations in the scientific literature highlighting great success

• Contents
• Master mix
• Positive control
• Water