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Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, without competent cells, 25 Reactions

Out of stock
Brand origin : United States
Expiry time : On demand
Delivery time : 1 Week/s
Delivery cost : On demand
Unit size : 25 Reactions
Order number : 450641
($) On demand

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, without competent cells, 25 Reactions


TOPO™ TA Cloning® Kits for Subcloning provide a highly efficient, 5-minute, one-step cloning strategy ('TOPO™ cloning') for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for subcloning. Each kit uses the pCR™ 2.1-TOPO™ TA vector with convenient restriction sites for subcloning. The cloning kits are available with a variety of competent cells, or without competent cells, depending upon your needs and budget.


Features

  • Fast and easy—go from PCR to clones in just 3 steps and in as little as 5 minutes hands-on time
  • Efficient—obtain up to 95% clones with correct insert
  • Proven—reliable performance for over a decade with over 4,000 citations
  • Simple—no ligase, post-PCR procedures, or PCR primers containing specific sequences are required


Contents

  • Topoisomerase I-activated pCR™2.1-TOPO™ vector
  • PCR buffer
  • Salt solution
  • dNTPs
  • Control template
  • M13 forward and reverse primers
  • Control PCR primers
  • Sterile water
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