Invitrogen™ pcDNA™3.1/V5-His TOPO™ TA Expression Kit, 20 reactions

Invitrogen™ pcDNA™3.1/V5-His TOPO™ TA Expression Kit, 20 reactions

The pcDNA™3.1/V5-His TOPO® TA Expression Kit offers one-step cloning of Taq-amplified PCR products into a high-level expression vector. Topoisomerase activation of the pcDNA3.1/V5-His-TOPO® vector allows PCR products to be ligated in just 5 minutes on your bench top and results in 90% recombinants.

Features

• Strong CMV promoter for high-level, constitutive expression.
• C-terminal V5 epitope tag for efficient detection of recombinant proteins with an Anti-V5 antibody.
• C-terminal polyhistidine (6xHis) sequence for purification using nickel-chelating resin and detection with an Anti-His (C-term) antibody.

Contents

• 20 µL pcDNA™3.1/V5-His-TOPO™ (10 ng/µL)
• 100 µL 10X PCR Buffer
• 10 µL dNTP Mix (50 mM)
• 50 µL Salt Solution
• 1 mL Sterile Water
• 10 µL Control PCR Template (50 ng/µL
• 10 µL Control PCR Primers (100 ng/µL each)
• 20 µL T7 Sequencing Primer (100 ng/µL)
• 20 µL BGH Reverse Sequencing Primer (100 ng/µL)
• 10 µL Expression Control Plasmid (500 ng/µL)
• 21 × 50 µL TOP10 E. coli cells
• 50 µL pUC19 Control DNA (10 pg/µL)
• 6 mL S.O.C. Medium