Invitrogen™ Champion™ pET300/NT-DEST and pET301/CT-DEST Gateway™ Vector Kit

Invitrogen™ Champion™ pET300/NT-DEST and pET301/CT-DEST Gateway™ Vector Kit

The Champion™ pET300/NT-DEST and pET301/CT-DEST Gateway® Vector Kit is designed for rapid cloning with a Gateway® entry clone and subsequent high-level prokaryotic expression controlled by the strong bacteriophage T7 promoter. In addition to the T7 promoter, each vector contains only the necessary functional elements and an N- or C-terminal 6xHis tag (pET300/NT-DEST and pET301/CT-DEST, respectively) for convenient purification and detection .

The vector kit is ideal for structural biologists who desire no or minimal modifications to their protein of interest. To maximize expression, use with MagicMedia™ E. coli Expression Medium.

Contents 

• The Champion™ pET300/NT-DEST 
• pET301/CT-DEST Gateway® Vector Kit includes 6 µg each of pET300/NT-DEST and pET301/CT-DEST vectors 
• 10 µg of control vector.