Invitrogen™ pcDNA™5/FRT/TO Vector Kit

Invitrogen™ pcDNA™5/FRT/TO Vector Kit

pcDNA™FRT⁄TO vector is a 5.1 kb inducible expression vector designed for use with the Flp-In™ T-REx™ System. The control plasmid, pcDNA™5⁄FRT⁄TO⁄CAT, is also included for use as a positive control for transfection and expression in your Flp-In™ T-REx™ host cell line of choice.

The pcDNA™5⁄FRT⁄TO vector contains the following elements:

• A hybrid human cytomegalovirus (CMV)⁄TetO2 promoter for high-level, tetracycline-regulated expression of the gene of interest in a wide range of mammalian cells
• Multiple cloning site with 10 unique restriction sites to facilitate cloning the gene of interest
• FLP Recombination Target (FRT) site for Flp recombinase-mediated integration of the vector into the Flp-In™ T-REx™ host cell line
• Hygromycin resistance gene for selection of stable cell lines

Contents

• 20 µg of each: pcDNA™FRT⁄TO and pcDNA™FRT⁄TO⁄CAT vectors.