Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1R E. coli

Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1R E. coli

The pCR™8/GW/TOPO® TA Cloning Kit delivers fast, efficient and reliable cloning. Additionally, this vector is ideal for use in Gateway® recombination cloning into various destination vectors for a multitude of applications.

Features

• Fast, 5-minute TOPO® TA Cloning delivers 95% recombinants
• 3´-T overhangs simplify cloning of Taq-amplified PCR products
• Fast growing MachI cells shorten cloning time
• Compatible with Gateway® recombination cloning enabling access to a multitude of destination vectors
• Sequencing primer sites located close to cloning insertion site so you sequence more insert, less vector the first time
• Spectinomycin resistance gene for robust selection in E. coli
• EcoR I sites flanking the PCR product insertion site for easy excision of inserts

Contents

• TOPO™-adapted, ready-to-use pCR™8/GW/TOPO™ Vector
• dNTPs
• Salt Solution
• Sterile Water
• Gateway Forward and Reverse Sequencing Primers
• Control Template and PCR Primers
• One Shot™ Mach1™-T1R Chemically Competent E. coli
• S.O.C. Medium
• pUC19 control plasmid