Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1R E. coli
On demand
Order number :
K252020
Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1R E. coli
The pCR™8/GW/TOPO® TA Cloning Kit delivers fast, efficient and reliable cloning. Additionally, this vector is ideal for use in Gateway® recombination cloning into various destination vectors for a multitude of applications.
Features
- Fast, 5-minute TOPO® TA Cloning delivers 95% recombinants
- 3´-T overhangs simplify cloning of Taq-amplified PCR products
- Fast growing MachI cells shorten cloning time
- Compatible with Gateway® recombination cloning enabling access to a multitude of destination vectors
- Sequencing primer sites located close to cloning insertion site so you sequence more insert, less vector the first time
- Spectinomycin resistance gene for robust selection in E. coli
- EcoR I sites flanking the PCR product insertion site for easy excision of inserts
Contents
- TOPO™-adapted, ready-to-use pCR™8/GW/TOPO™ Vector
- dNTPs
- Salt Solution
- Sterile Water
- Gateway Forward and Reverse Sequencing Primers
- Control Template and PCR Primers
- One Shot™ Mach1™-T1R Chemically Competent E. coli
- S.O.C. Medium
- pUC19 control plasmid
There are no specifications
There are no report
You May Also Like
![](https://laboshop.ae/public/storage/files/42otVhri4xm5h9lxhA0lhaoqjv8aZrI5LD9VwkTk.png)
Invitrogen™ pENTR™/D-TOPO™ Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli
(Ex-work price)
$ On demand
$ On demand
On demand
![](https://laboshop.ae/public/storage/products/September2021/SaqC5ZfTYlCf9PghzW4C.jpg)
Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with One Shot™ MAX Efficiency™ DH5α-T1R E. coli, 50 Reactions
(Ex-work price)
$ On demand
$ On demand
On demand
![](https://laboshop.ae/public/storage/files/743dae0b-9bcf-4452-b390-f64dc0caf38c.png)
Invitrogen™ BLOCK-iT™ Lentiviral Pol II miR RNAi Expression System with EmGFP
(Ex-work price)
$ On demand
$ On demand
On demand
![](https://laboshop.ae/public/storage/products/September2021/W11xPt3gdPS1uUNKutXZ.jpg)
Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ TOP10 chemically competent E. coli cells, 25 Reactions
(Ex-work price)
$ On demand
$ On demand