Invitrogen™ PCR Cloning System with Gateway™ Technology with pDONR™221 & OmniMAX™2 Competent Cells

Invitrogen™ PCR Cloning System with Gateway™ Technology with pDONR™221 & OmniMAX™2 Competent Cells

Gateway® Technology enables rapid cloning of one or more genes into virtually any protein expression system. Once you have an entry clone, you can recombine your gene of interest into a variety of expression vectors adapted for use in Gateway® Technology. The PCR Cloning System with Gateway® Technology is designed to create an entry clone following PCR with attB-modified custom primers (Figure 1). The PCR product is directionally cloned with efficiencies typically >99%. 

Features

Rapid, efficient, directional cloning of PCR products

Versatility to use any amplification enzyme

Cloning without the need for restriction endonucleases or ligase

Selection of entry clones with kanamycin or Zeocin™

Contents

• The PCR Cloning Kit with Gateway™ Technology includes Gateway™ BP Clonase™ II enzyme mix and reagents
• Gateway™ pDONR™221
• M13 sequencing primers
• PEG/Mg2+ solution
• proteinase K
• pEXP7-tet positive control
• One Shot™ OmniMAX™2 Competent Cells. 

In addition, the pDONR™221 and pDONR™/Zeo vectors have a pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use.