Invitrogen™ pEF6/V5-His TOPO™ TA Expression Kit

Invitrogen™ pEF6/V5-His TOPO™ TA Expression Kit

The pEF6/V5-His TOPO® TA Expression Kit is designed for high-level mammalian expression from the powerful human EF-1 α promoter with the added convenience of one-step TOPO® Cloning. The pEF6/V5-His-TOPO® vector includes:

Enhancer/promoter elements from the human elongation factor 1α subunit (hEF-1α) for high-level expression in mammalian cells

Features

• The Blasticidin resistance gene for rapid selection of stable mammalian cell lines
• A C-terminal V5 epitope for efficient detection with an Anti-V5 Antibody
• A C-terminal polyhistidine (6xHis) sequence for rapid purification with nickel-chelating resin and detection with an Anti-His(C-term)
• Antibody

Contents

• The TOPO™ TA box contains all of the reagents necessary for ligation, including 200 ng of topoisomerase I-activated pEF6/V5-His-TOPO™ vector
• sterile water
• dNTPs
• 10X PCR buffer
• salt solution
• control templates and primers
• primers for sequencing or PCR screening
• an expression control plasmid
• The One Shot™ Competent Cell box contains all of the reagents necessary for transformation, including twenty-one 50-µl aliquots of TOP10 Chemically Competent E. coli
• S.O.C. medium
• pUC19 supercoiled control plasmid.