Invitrogen™ Anza™ T4 DNA Ligase Master Mix, 50 Reactions
On demand
Order number :
IVGN2104
Invitrogen™ Anza™ T4 DNA Ligase Master Mix, 50 Reactions
The Invitrogen Anza™ T4 DNA Ligase Master Mix facilitates the joining of abutted 5’-phosphate and 3’-hydroxyl termini in duplex DNA through the formation of a phosphodiester bond. It can be used to join DNA fragments with both sticky ends and blunt ends, and to repair nicks in double-stranded DNA with 3'-hydroxyl and 5'-phosphate ends. Anza T4 DNA Ligase is formulated as a 4X concentrated master mix. Ligation can be performed with DNA in water, TE, elution buffer, or 1X Anza™ buffers.
Features
- Ligation complete in 15 minutes at room temperature
- A single ligase master mix for both sticky-end and blunt-end ligation reactions
- Ready-to-use master mix format reduces pipetting steps
- 4X concentration enables more dilute DNA to be used in ligation reaction without concentrating
Contents
- 1 mL Anza T4 DNA Ligase Master Mix
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