Invitrogen™ GeneArt™ High-Order Genetic Assembly System

Invitrogen™ GeneArt™ High-Order Genetic Assembly System

The GeneArt® High-Order Genetic Assembly System is a highly efficient kit for the simultaneous and seamless assembly of up to 10 DNA fragments, totaling up to 110 Kbp in length, into any vector. The system relies on yeast’s ability to take up and recombine DNA fragments with high efficiency. This greatly reduces the in vitro handling of DNA and eliminates the need for enzymatic treatments, such as restriction and ligation, while allowing for precise fusions of DNA sequences. The kit contains materials for the transformation and purification from yeast (no growth media), and competent E. coli for plasmid amplification of correct clones.

Features

• Easy and Powerful — Clone up to 10 DNA fragments, with the sequence of your choice, simultaneously in a single vector (up to 110 Kbp); no restriction digestion, ligation or recombination sites required
• Precision and Efficiency —Designed to let you clone what you want, where you want, in the orientation you want, and achieve up to 90% correct clones with no extra sequences left behind
• Flexibility — Use our linear vector, a vector of your choice, or clone pre-existing DNA fragments that have no end-homology without further modifications
• Free Tools — Design DNA oligos and more with our free web-based interface that walks you through your project step-by-step
• Diverse Applications — Streamline many synthetic biology and molecular biology techniques through the rapid combination, addition, deletion, or exchange of DNA segments

Contnets

• One Shot™ MaV203 yeast competent cells
• One Shot™ TOP10 Electrocompetent E.coli cells
• PEG⁄Lithium Acetate solution
• S.O.C. medium
• Control cloning vector and insert for DNA assembly
• Supercoil vector controls for transformation
• Lysis buffer and glass beads