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Invitrogen™ pENTR™/SD/D-TOPO™ Cloning Kit, with One Shot™ Mach1™-T1R Chemically Competent E. coli

On demand
Order number : K263520
(Ex-work price)
$ On demand

Invitrogen™ pENTR™/SD/D-TOPO™ Cloning Kit, with One Shot™ Mach1™-T1R Chemically Competent E. coli


The pENTR™/SD/D-TOPO® Cloning Kits utilize a highly efficient, 5-minute cloning strategy ("TOPO® Cloning") to directionally clone a blunt-end PCR product into a vector for entry into the Gateway® System or the MultiSite Gateway® System. Blunt-end PCR products clone directionally into the pENTR™/SD/D-TOPO® entry vector at greater than 90% efficiency, and include an upstream Shine-Dalgarno sequence for expression in prokaryotes.


Features

  • Gateway® System Ready - Rapidly shuttle cloned genes between multiple vector systems
  • Fast and Easy - Go from PCR to Gateway® Entry clone in just 3 steps and as little as ∼5 minutes hands on time
  • Efficient - Achieve over 90% clones with correct insert in the right direction
  • Proven - Reliable performance for over a decade


Contents

  • Each Directional TOPO™ pENTR Cloning Kit contains the appropriate, ready-to-use TOPO™ vector
  • dNTPs
  • salt solution
  • sterile water
  • universal M13 sequencing primers
  • TOP10 or Mach1-T1R Chemically E. coli
  • S.O.C. Medium
  • a pUC19 control plasmid
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