Invitrogen™ pENTR™/SD/D-TOPO™ Cloning Kit, with One Shot™ Mach1™-T1R Chemically Competent E. coli
On demand
Order number :
K263520
Invitrogen™ pENTR™/SD/D-TOPO™ Cloning Kit, with One Shot™ Mach1™-T1R Chemically Competent E. coli
The pENTR™/SD/D-TOPO® Cloning Kits utilize a highly efficient, 5-minute cloning strategy ("TOPO® Cloning") to directionally clone a blunt-end PCR product into a vector for entry into the Gateway® System or the MultiSite Gateway® System. Blunt-end PCR products clone directionally into the pENTR™/SD/D-TOPO® entry vector at greater than 90% efficiency, and include an upstream Shine-Dalgarno sequence for expression in prokaryotes.
Features
- Gateway® System Ready - Rapidly shuttle cloned genes between multiple vector systems
- Fast and Easy - Go from PCR to Gateway® Entry clone in just 3 steps and as little as ∼5 minutes hands on time
- Efficient - Achieve over 90% clones with correct insert in the right direction
- Proven - Reliable performance for over a decade
Contents
- Each Directional TOPO™ pENTR Cloning Kit contains the appropriate, ready-to-use TOPO™ vector
- dNTPs
- salt solution
- sterile water
- universal M13 sequencing primers
- TOP10 or Mach1-T1R Chemically E. coli
- S.O.C. Medium
- a pUC19 control plasmid
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