Invitrogen™ Permeabilization Medium (Medium B), 100 mL

Invitrogen™ Permeabilization Medium (Medium B), 100 mL

Permeabilization Medium (Medium B) is a component of the FIX & PERM® Cell Permeabilization Kit. The kit is used in flow cytometry for fixing and permeabilizing cells in suspension, allowing access of antibody to intracellular targets without sacrificing scatter characteristics.

Features

• Flexible—compatible with multiple sample types and cellular antigens
• Robust—scatter pattern of cells unaffected
• Efficient—simultaneous detection of intracellular and surface markers

The Permeabilization Medium formula was specifically designed to reduce background staining and allow simultaneous addition of fluorochrome-labeled antibodies during the permeabilization step.

How It Works

Cells are incubated for 15 minutes at room temperature with Fixation Medium (Medium A) (Cat. No. GAS001S100) and washed. Cells are then incubated with the Permeabilization Medium and fluorochrome-labeled antibody. Both Fixation Medium and Permeabilization Medium are included in the FIX & PERM® Cell Permeabilization Kit.