Invitrogen™ pHrodo™ Green S. aureus BioParticles™ Phagocytosis Kit for Flow Cytometry

Invitrogen™ pHrodo™ Green S. aureus BioParticles™ Phagocytosis Kit for Flow Cytometry

The pHrodo™ Green S. aureus BioParticles™ Phagocytosis Kit for Flow Cytometry enables the detection of phagocytic activity in whole blood samples and live cell lines by flow cytometry. The kit includes all the reagents required for assessing particle ingestion and red blood cell lysis. Sufficient reagents are provided in the kit for performing approximately 100 assays using 100 µL of whole blood per assay.

Features

• The pHrodo™ Green Dye, The new Molecular Probes™ proprietary, pH-sensitive, pHrodo™ Green dye is non-fluorescent at neutral pH, but turns bright green upon acidification. Because it is both fluorogenic and pH-sensitive, the pHrodo™ Green dye can be used as a specific sensor of phagocytic events; acidification of the phagosome following phagocytosis is marked by green fluorescence. It is therefore an ideal tool with which to study phagocytosis and its regulation by drugs and/or environmental factors.
• Flexible—multiplex with reagents excited by other laser lines, The fluorophore is readily excited with the 488 nm argon-ion laser and fluorescence emission can be collected using a standard 530/30 nm BP filter on most flow cytometers, and thus is easily multiplexable with reagents used on other lasers .
• Rapid and reproducible, Wash steps and quencher dyes are not needed, since the pHrodo™ Green dye is non-fluorescent outside the cell, making the staining protocol simple and fast. The elimination of wash steps and quencher dyes also improves assay reproducibility, particularly in plate reader-based assays.
• Use with bacteria, Analysis of phagocytosis by Gram-positive or Gram-negative bacteria is easily accomplished using the ready-made pHrodo™ Green BioParticles™ conjugates. Use ready-made pHrodo™ Green BioParticles™ conjugates for convenient analysis of phagocytosis of Gram-positive or Gram-negative bacteria, or the amine-reactive pHrodo™ Green STP ester for labeling microorganisms or proteins of your choice.

Contents

• lysis buffer (10 mL).
• Buffer B (200 mL).
• wash buffer (200 mL).
• pHrodo™ green S. aureus BioParticles™ (5 vials, lyophilized product).